Factors influencing Agrobacterium-mediated embryogenic callus transformation of Valencia sweet orange (Citrus sinensis) containing the pTA29-barnase gene
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چکیده
منابع مشابه
A b-galactosidase gene is expressed during mature fruit abscission of ‘Valencia’ orange (Citrus sinensis)
b-galactosidases have been detected in a wide range of plants and are characterized by their ability to hydrolyse terminal non-reducing b-D-galactosyl residues from b-D-galactosides. These enzymes have been detected in a wide range of plant organs and tissues. In a search for differentially expressed genes during the abscission process in citrus, sequences encoding b-galactosidase were identifi...
متن کاملA beta-galactosidase gene is expressed during mature fruit abscission of 'Valencia' orange (Citrus sinensis).
beta-galactosidases have been detected in a wide range of plants and are characterized by their ability to hydrolyse terminal non-reducing beta-D-galactosyl residues from beta-D-galactosides. These enzymes have been detected in a wide range of plant organs and tissues. In a search for differentially expressed genes during the abscission process in citrus, sequences encoding beta-galactosidase w...
متن کاملAgrobacterium - Mediated Transformation of Citrus Cell Suspension Cultures
An efficient protocol for the genetic transformation of cell suspension cultures of several citrus cultivars using Agrobacterium is described. Cell suspension cultures of Citrus sinensis ‘Hamlin’, ‘Valencia’ and ‘OLL8’ (an early maturing ‘Valencia’ like somaclone), Citrus unshiu ‘Okitsu wase’ and Citrus reticulata ‘Ponkan’ and ‘W. Murcott’ were transformed using A. tumefaciens EHA105. It was de...
متن کاملPhotosynthetic Activity in the Flower Buds of ;Valencia' Orange (Citrus sinensis [L.] Osbeck).
Flower buds of ;Valencia' orange (Citrus sinensis [L.] Osbeck) were able to fix (14)CO(2) into a number of compounds in their own tissues under both light and dark conditions. The total incorporation, however, was about 4-fold higher in the light than in the dark. In the light, 50% of the total (14)C label was found in the neutral fraction (sugars), 22% in the basic fraction (amino acids), and ...
متن کاملEffect of Water Stress on Ethylene Production by Detached Leaves of Valencia Orange (Citrus sinensis Osbeck).
Detached leaves of Valencia oranges, Citrus sinensis Osbeck, emanated ethylene at markedly higher rates and contained more endogenous ethylene when placed under water stress at 55% relative humidity than when placed in water-saturated air. Water stress induced defoliation from detached branches. Relieving the water stress of such leaves by transfer to a mist chamber resulted in lowering of the ...
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ژورنال
عنوان ژورنال: Tree Physiology
سال: 2003
ISSN: 0829-318X,1758-4469
DOI: 10.1093/treephys/23.17.1209